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human foreskin fibroblast bj 5ta  (ATCC)


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    ATCC human foreskin fibroblast bj 5ta
    Human Foreskin Fibroblast Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+fibroblast+bj/BJ-5ta%3B+Foreskin+Fibroblast%3B+Human/10__3390_slash_molecules31050862-101-0-4
    Average 96 stars, based on 161 article reviews
    human foreskin fibroblast bj 5ta - by Bioz Stars, 2026-10
    96/100 stars

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    Cell Culture:

    Article Title: Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction.
    Article Snippet: Schematic of SMCHD1 as a host restriction factor for AAV transduction. https://doi.org/10.1371/journal.ppat.1012344.g007 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1012344 July 8, 2024 15 / 28 and antibiotics (penicillin 100U/mL, Streptomycin 100 μg/mL, Amphotericin B 250 ng/mL) (Solarbio, P7630) and passaged at 80–90% confluence using 0.25% Trypsin-EDTA (Gibco, 25200072). .. Human foreskin fibroblast BJ (ATCC, CRL-2522) and HepG2 (MeisenCTCC, CTCC-001-0014) were cultured in DMEM supplemented with 10% FBS and antibiotics (penicillin 100 U/mL, Streptomycin 100 μg/mL, Amphotericin B 250 ng/mL) and passaged at a ratio of 1:4 every three days using 0.25% Trypsin-EDTA. ..

    Article Title: Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction
    Article Snippet: HeLa, HeLa derived SMCHD1-KO cells, 293FT (Invitrogen, R7007), and HEK293 cells (ATCC, CRL-1573) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, C11995500BT) supplemented with 10% fetal bovine serum (FBS) (PAN-Biotech, ST30-3302) and antibiotics (penicillin 100U/mL, Streptomycin 100 μg/mL, Amphotericin B 250 ng/mL) (Solarbio, P7630) and passaged at 80–90% confluence using 0.25% Trypsin-EDTA (Gibco, 25200072). .. Human foreskin fibroblast BJ (ATCC, CRL-2522) and HepG2 (MeisenCTCC, CTCC-001-0014) were cultured in DMEM supplemented with 10% FBS and antibiotics (penicillin 100 U/mL, Streptomycin 100 μg/mL, Amphotericin B 250 ng/mL) and passaged at a ratio of 1:4 every three days using 0.25% Trypsin-EDTA. ..



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    Image Search Results


    (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing

    Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: DNA Methylation Assay, CRISPR, Nanopore Sequencing, Methylation